Biomembranes, Part V: Cellular and Subcellular Transport: by John N. Abelson, Melvin I. Simon, Sidney Fleischer, Becca

By John N. Abelson, Melvin I. Simon, Sidney Fleischer, Becca Fleischer

The delivery volumes of the Biomembranes sequence have been initiated with Volumes one hundred twenty five and 126 of tools in Enzymology, which lined delivery in micro organism, Mitochondria, and Chloroplasts. Volumes 156 and 157 endured the subject matter with ATP-Driven Pumps and similar delivery. mobile and Subcellular shipping: Eukaryotic (Nonepithelial) Cells was once the subject of Volumes 173 and 174. The subject matter of this quantity, in addition to of quantity 192, is mobile and Subcellular delivery: Epithelial Cells.

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Extra info for Biomembranes, Part V: Cellular and Subcellular Transport: Epithelial Cells, Volume 191 (Methods in Enzymology)

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The problem associated with reducing the pressure of perfusion is that the flow of perfusate, and hence the delivery of nutrients and oxygen to the renal tubular cells, may become rate limiting. A much better approach is to increase the oncotic pressure of the perfusate. This can be readily accomplished by perfusing the kidney with a concentration of bovine serum albumin calculated to provide an oncotic pressure that is high enough to counterbalance the filtration pressure at the glomerulus. Because the flow of perfusate through the isolated kidney perfused with an artificial solution is generally very high, of the order of 20-22 ml/min/g, there is little or no decline in the pressure of filtration along the glomerular capillary (in the filtering kidney this translates into a very low filtration fraction, of the order of 2 to 3%, and an absence of filtration pressure equilibrium; in other words, there is filtration all along the glomerular capillary).

Physiol. 232, F448 (1977). 37 W. S. Marshall and S. D. Klycc, J. Membr. Biol. 73, 275 (1983). 3s N. K. Wills and C. Clauscn, J. Membr. Biol. 95, 21 (1987). 39 R. Rick, A. D6rgc, and E. Sessclmann, PfluegersArch. 411, 243 (1988). [2] PARACELLULAR SHUNT CONDUCTANCE IN EPITHELIA 15 In Appendix B we report some model calculations which define the validity limits of the method quantitatively. From this we deduce that the method is restricted to the simple model of tight Na+-absorbing epithelia in which R , is exclusively selective for Na + and to the use of ionophores that are also purely selective for Na +.

The filters used are Millipore type LC l0 g m with a prefilter type AP Cat. #AP20 02200. Both filters are contained in stainless steel holders. The second pump takes the perfusate from the bottom of the oxygenator to the kidney. The arterial line has an in-line Brooks flowmeter with a tantalum float, the only float that is capable of reading the large flows found in this preparation. The arterial line also has a pressure gauge attached to it by a T-type connector. The arterial line has in addition a derivation to the reservoir.

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