Calculating the Secrets of Life: Applications of the by Eric S. Lander, Michael S. Waterman

By Eric S. Lander, Michael S. Waterman

During this first-ever survey of the partnership among arithmetic and biology, prime specialists examine how mathematical learn and strategies have made attainable vital discoveries in biology. Explores how differential geometry, topology, and differential mechanics have allowed researchers to "wind" and "unwind" DNA's double helix to appreciate the phenomenon of supercoiling. Explains how mathematical instruments are revealing the workings of enzymes and proteins. Describes how mathematicians are detecting echoes from the foundation of lifestyles via employing the stochastic and statictical conception to the examine of DNA sequences.

Show description

Read Online or Download Calculating the Secrets of Life: Applications of the Mathematical Sciences in Molecular Biology PDF

Similar molecular biology books

An Introduction to Genetic Engineering (Studies in Biology)

The writer offers a easy advent to the realm of genetic engineering. a few two decades have handed because the first recombinant DNA molecules have been developed at Stanford collage. Genetic engineering is now a truth and this publication easily and concisely describes to the coed the complete diversity of permitting applied sciences to be had.

Transformations of Lamarckism: From Subtle Fluids to Molecular Biology (Vienna Series in Theoretical Biology)

In 1809 -- the 12 months of Charles Darwin's delivery -- Jean-Baptiste Lamarck released Philosophie zoologique, the 1st accomplished and systematic conception of organic evolution. The Lamarckian strategy emphasizes the new release of developmental diversifications; Darwinism stresses choice. Lamarck's rules have been finally eclipsed by way of Darwinian techniques, particularly after the emergence of the fashionable Synthesis within the 20th century.

Handbook of Epigenetics: The New Molecular and Medical Genetics

Epigenetics is taken into account by means of many to be the "new genetics" due to the overwhelming facts of the contribution of non-genetic elements equivalent to foodstuff, setting, and chemical publicity on gene expression. the consequences of epigenetics are immense, together with tissue/organ regeneration, X-chromosome inactivation, and stem cellphone differentiation and genomic imprinting and getting older.

Molecular Biology of the Cell 6E - The Problems Book

The issues publication is helping scholars savor the ways that experiments and straightforward calculations may end up in an realizing of ways cells paintings through introducing the experimental starting place of mobile and molecular biology. every one bankruptcy studies keyword phrases, assessments for knowing simple ideas, and poses research-based difficulties.

Additional resources for Calculating the Secrets of Life: Applications of the Mathematical Sciences in Molecular Biology

Sample text

Components of an ECM System and Important Parameters Generally, ECM systems consist of a generator providing the electric signals and a chamber in which the cells are subject to the electric fields created by the voltage pulse from the generator. A third optional component is a monitoring system, either built into the generator or connected in line between the generator and the chamber, which measures the electrical parameters as the pulse passes through the system. -6. In this section, we discuss the relationship between the electrical parameters, which the ECM system provides, and the parameters that the cells experience.

The above analysis applies only to reversible breakdown of cell membranes by the electric pulses, such that membranes reseal in time, and cells recover from the traumatic event of electroporation. , E >> Eb such that r > rC, electropores do not reseal, and the cell viability is low. As a consequence, cell viability imposes an upper limit on the transfection efficiency. 3. Experimental Evidence Several experiments using fluorescent molecules as tracers have shown that macromolecule leakage or intake during electroporation is indeed proportional to the quantity (E - Eb)T (4,12,13).

Day 1. Introduction Proteins can be introduced into cells in several ways, including permeabilization by Sendai virus, trypsinization, osmotic shock, microinjection, electroporation, or after transfection of cells with expression vectors containing the gene(s) of interest. Unlike most of these other methods, electroporation is a simple, relatively inexpensive, and efficient method for rapidly introducing DNA, small molecules, and proteins into bacterial or mammalian cells (l-10). Electroporation has the advantage over other techniques in that many cells can be treated simultaneously and one or more compounds or macromolecules, such as proteins, can be introduced into cells at the same time (see Note 1).

Download PDF sample

Rated 4.29 of 5 – based on 43 votes