By Barbara J. Bain
UP-TO-DATE sensible consultant TO LEUKAEMIA DIAGNOSIS
Written via a well known professional this functional consultant were totally revised and up-to-date. The e-book covers contemporary advances within the fields of immunophenotyping, cytogenetics and molecular genetics. It illustrates how laboratory strategies are used for the analysis and category of leukaemia and comprises pictures of irregular cells to assist diagnosis.
This 5th edition:
- Incorporates the ideas of the 2015 WHO class of Tumours of Haematopoietic and Lymphoid Tissues
- Includes three hundred top of the range complete color electronic photographs of irregular cells in leukaemia and lymphoma
- Full color diagrams and beneficial tables make clear and summarise key issues throughout
Essential analyzing for each haematologist and haematopathologist.
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Additional info for Leukaemia Diagnosis
10 Criteria for the diagnosis of acute myeloid leukaemia of M7 category (acute megakaryoblastic leukaemia). Blasts ≥30% of bone marrow nucleated cells Blasts demonstrated to be megakaryoblasts by immunological markers, ultrastructural examination or ultrastructural cytochemistry AML shows a markedly increased incidence in children with Down’s syndrome. In infants and children, M7 AML may be associated with t(1;22)(p13;q13) (see page 109) and, in adults, a signiﬁcant proportion of cases are associated with abnormalities of chromosome 3.
MGG × 348. (a) Fig. 50 BM ﬁlm from a patient with acute leukaemia showing mast cell and neutrophilic differentiation; (a) blast cells and immature abnormal mast cells; (b) abnormal mast cells and a blast cell containing an Auer rod (courtesy of Dr Neelam Varma, Chandigar). MGG × 870. (b) 40 Chapter 1 Fig. 51 BM ﬁlm from a patient with acute mast cell leukaemia showing a mature mast cell packed with granules that are strongly positive for mast cell tryptase and several blast cells with tryptase-positive granules; these latter cells are therefore identiﬁed as blast cells of mast cell lineage (same patient as Fig.
Such cases may occur de novo but it is likely that cases resembling M5 AML supervening in Langerhans’ cell histiocytosis  also represent a leukaemia of Langerhans’ cells. The diagnosis is made by assessment of cytology and immunophenotype (CD1a is expressed) with the demonstration of Birbeck granules by ultrastructural examination providing a deﬁnitive diagnosis. A case of AML showing features of dendritic cell differentiation in vitro has also been reported; it resembled M2 AML cytologically and cytochemically .