New Diagnostic Methods in Oncology and Hematology, 1st by J. Oertel, D. Huhn (auth.), Professor Dr. med. Dieter Huhn

By J. Oertel, D. Huhn (auth.), Professor Dr. med. Dieter Huhn (eds.)

This e-book describes the newest tools in oncological and hematological diagnostics, together with immunological, molecular genetic and cytogenetic assays. All tools are defined of their various adaptations and in comparison by way of rate and effectiveness. each one bankruptcy ends with an in depth "how-to-do" part. The ebook is written for scientists, clinicians and group of workers from examine and from regimen laboratories. It satisfies the expanding call for for info on new equipment in hematology and oncology.

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APAAP immunostaining (anti-kappa) of a cytospin (mononuclear blood cells) from a patient with B-CLL Fig. loll. APAAP immunostaining (anti-lambda) of a cytospin from the same patient as in Fig. 10 Fig. 12. APAAP immunostaining (anti-CD19) of a bone marrow smear from a patients with marrow infiltration by a small cell B-non-Hodgkin's lymphoma Fig. 13. May-Griinwald-Giemsa staining of mononuclear blood cells from a patient with large cell B-lymphoma; ~ centroblast Fig. 14. APAAP immunostaining (anti-kappa); same patient as in Fig.

A strict follow-up of such patients is, however, necessary. The analysis of lymph node aspirates is useful for detection of recurrent malignant tumor. 4 Infectious Disorders Immunochemistry is an important tool for diagnosis of several infectious disorders such as cytomegalovirus (CMV) infection. Traditionally, infectious agents were identified in the microbiology laboratory by culture and serological technique. However, some infectious agents can be difficult to culture and serology results can be difficult to interpret from patients with immunodeficiency.

Naptophysin, thyreoglobulin, vimentin and von Willebrand factor. 5 Protocol V: Enhanced Labeled-Polymer System (ELPS, Envision) [10) Specimens: cytospins from blood, bone marrow, serous effusions, cerebrospinal fluid, fine needle aspirates, bronchoalveolar lavage; smears from blood, bone marrow or fine needle aspirates Performance: in coverplates (Shandon), in a humid chamber or in an automatic staining procedure Immunostaining: Fixation with acetone (100%) Primary antibody(mouselrabbit anti-human)b Dilution 10 min 30-60 min titration 1:10-1000 Wash with TBS' ready to use Polymer conjugated with goat 15-30 min anti-mouse/rabbit antibody andalkaline phosphatase (DAKO K4017) Wash with TBS 15 min Alkaline phosphatase detection c Wash with TBS and then with water 5 min Nuclear staining (hemalum, Merck No.

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